产品详情
产品名称Cox2 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC IF
种属反应性Hu Ms Rt
特异性The antibody detects endogenous levels of total Cox2 protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from human Cox2.
基因/蛋白名称Cox2
别名COX2; GRIPGHS; PGHS2; CYCLOOXYGENASE 2; GLUCOCORTICOID-REGULATED INFLAMMATORY PROSTAGLANDIN G/H SYNTHASE
数据库入口号Swiss-Prot: P35354
NCBI Gene ID: 5743
UniprotP35354
实际分子量80kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cox2 antibody #33345.
Immunofluorescence analysis of A549 cells, using Cox2 antibody #33345.
Western blot analysis of extracts from A549 cells, using Cox2 antibody #33345.
Western blot analysis of extracts from HeLa cells (Lane 2), and A549 cells (Lane 3), using Cox2 antiobdy #33345. The lane on the left is treated with systhesized peptide.
背景
Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Nathalie Bonnefoy, Mol. Cell. Biol., Apr 2001; 21: 2359 - 2372.
Keith P. Choe, J. Exp. Biol., May 2006; 209: 1696 - 1708
D. Shelley Lupold, PNAS, Sep 1999; 96: 11670.
Axel J. Baumgarten, AACR Meeting Abstracts, Mar 2004; 2004: 1078.
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